By A. L. J. Gielkens, A. J. M. Berns (auth.), G. Wittmann, S. A. Hall (eds.)

Aujeszky's illness (AD) is expanding in Europe and it has turn into a major challenge in many of the international locations of the eu groups (EC). The keep watch over and eradication of the ailment is particularly tricky due to the fact that advert virus (ADV) inspires a persistant latent an infection in its major host, the pig. Such latent an infection may also take place while vaccinated pigs are uncovered to the virus. In view of this, the fee of the eu groups (CEC) suggestion it essential to have a survey at the present kingdom of data on advert and ADV. as a result, a seminar used to be organised by means of the Federal study Institute for Animal Virus ailments in Tubingen, Federal Republic of Germany, and held there on June nine and 10, 1981. The seminar used to be part of the 'Animal Pathology Programme' of the CEC. The seminar used to be attended via forty four members from the international locations of the EC, and 29 papers have been awarded which coated a large box of study on advert: homes of the virus, diagnostic techniques, immunity and pathogenesis, vaccination, latent an infection, epidemiology, keep watch over and eradication. Scientists from fluctuate­ ent institutes within the EC who've been engaged on advert therefore had the oppor­ tunity to replace their wisdom in addition to to offer and obtain impetus for extra clinical paintings. additionally, many information got that are of curiosity for the veterinary professionals in regards to regulate and eradication of AD.

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Additional resources for Aujeszky’s Disease: A Seminar in the Animal Pathology Series of the CEC Programme of Coordination of Agricultural Research, held at Tubingen, Federal Republic of Germany June 9–10, 1981

Example text

57 INFLUENCE OF VACCINATION WITH MODIFIED LIVE VACCINE ON THE LABORATORY DIAGNOSIS OF AUJESZKY'S DISEASE C. A. Pol Central Veterinary Institute, Virology Department, Houtribweg 39, 8221 RA Lelystad, The Netherlands. ABSTRACT Litters of two week-old piglets were vaccinated with commercially available modified live virus (MLV) vaccines and killed at intervals between 4 and 14 days post vaccination. Parts of the central nervous system, tonsils and lungs were collected for virus isolation and for immunofluorescent and histopatholog1:c studies.

Crossed immunoelectrophoresis in agarose gels. ADV cellular antigens were sUbjected to first dimension electrophoresis followed by second dimension electrophoresis into ADV specific polyvalent antibodies (15 ~1/cm2). , 1978). tested for the capacity to inactivate AOV . Three out of the four precipitation bands induced neutralising antibodies (Figure 3). The analysis of the antisera by the indirect immunoprecipitation technique revealed that all sera except one precipitated more than one glycoprotein.

IgG and IgM were pre- pared as described by Metzger and Fougereau (1967) and Curtis and Bourne (1971), respectively. VNA tests The virus strain DaS67 and primary pig kidney or primary or secondary pig testis cell monolayers in tissue culture tubes were used. Maintenance medium was Eagle's minimal essential medium with 2% heat-inactivated bovine serum. Hanks' salt solution with 2% serum as above was used for virus suspensions and serum dilutions. All sera were heat-inactivated at 56 o C. Incubation of virus-serum mixtures (preincubation) was performed at 37 0 C for 24 h (P37/24 test) if not mentioned otherwise.

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