By J. Bignon
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Additional info for Biochemistry, Pathology and Genetics of Pulmonary Emphysema. Proceedings of an International Symposium Held in Sassari, Italy, 27–30 April 1980
1 μπιοί of the chromatographically-pure d e s m o s i n e preparation w a s dissolved in water at I m M concentration and this sample served as a standard solution. T h e exact concentration of d e s m o s i n e in this standard w a s m e a s u r e d by a m i n o acid analysis with lysine as a calibration reference. ) M o l e s d e s m o s i n e in the standard were converted to μg d e s m o s i n e using a m o l e c u l a r weight of 5 2 6 daltons. Preparation of ^l-labelled desmosine A portion of the chromatographically-pure d e s m o s i n e preparation w a s labelled with 1Z5 Bolton-Hunter reagent ( I) according to m e t h o d s r e c o m m e n d e d by N e w E n g l a n d N u c l e a r .
M o l e c u l a r weights of the resulting bands were estimated by c o m p a r i s o n with bovine serum albumin, catalase, o v a l b u m i n , c h y m o t r y p s i n o g e n - A and R N A a s e standards. T h e protein content of the antigenic fractions in the detergent w a s d e t e r m i n e d by the modification of the L o w r y procedure described by DULLEY and GRIEVE [ 7 ] . E a c h concentration of the standard and of the test fractions w a s run in duplicate. T w o tubes w e r e set up as blanks with all the reagents but without s a m p l e or standard.
In : N e u t r a l p r o t e a s e s o f h u m a n p o l y m o r p h o n u c l e a r l e u k o c y t e s . K. H a v e m a n n and A . Janoff eds. U r b a n a n d S c h w a r zenberg, B a l t i m o r e - M u n i c h , 1 9 7 8 , p p . 1 6 7 - 1 7 8 . ). — Inhibition of elastase f r o m g r a n u l o c y t e s b y the l o w m o l e c u l a r w e i g h t bronchial protease inhibitor. Scand. J. clin. Lab. , 1976, 36, 437-445. ). — Cigarette s m o k i n g and the l u n g s : relation t o p o s t m o r t e m evidence of e m p h y s e m a , chronic bronchitis and b l a c k l u n g p i g m e n tation.